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Using fluorescently labelled anti-ErbB2 or anti-EGFR extracellular antibodies allowed us to measure cell surface ErbB2 and EGFR levels following POH1 knockdown by FACS analysis (Figure 7A,B).
We tested the effects of over expression of RACK1 in transiently expressing HEK cells by co-transfecting Flag-M2 with either PCDNA3.1 or RACK1 into HEK cells and used the membrane-impermeable antagonist ([3H]NMS) binding to measure cell surface receptors after 5, 15, 30 and 60 minutes of carbachol stimulation (Fig. 2B).
To measure cell surface VEGFR-3, a fraction of the lysates was precipitated with streptavidin agarose beads (Life Technologies).
To measure cell surface PTPRZ1 levels in sh Z1-transduced SCLC cells, we used flow cytometry (FACS).
NADH is an impermeant substrate and, therefore, can be used to measure cell surface NADH activity with whole cells.
To measure cell surface dissociation constants, cells were incubated with various concentrations of each probe and then stained with 2 μM of fluorogen.
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Polymorphonuclear neutrophil activation was analyzed by measuring cell surface and soluble cell adhesion molecule expressions.
We assessed class-switching of atypical MBCs by measuring cell surface expression of IgG by flow cytometry (Fig. 4).
In this context, measuring cell surface levels of MC1R is not an adequate estimation of the availability of this protein to the T cell system.
To assess whether the relative abundance of TRAIL receptors on the cell surface accounted for differences in sensitivity to TRAIL-induced apoptosis, we measured cell surface expression of TRAIL-R1, -R2, -R3 and -R4 by flow cytometry.
We provide evidence that cells sense a critical cell size by measuring cell surface area rather than, for example, cell volume or absolute length, a mechanism that could function regardless of the cell shape.
More suggestions(15)
measure cell viability
measure cell membrane
measure cell contraction
measure airway surface
measure cell line
measure cell apoptosis
measure cell staining
measure cell cycle
measure cell motility
measure coal surface
measure cell concentration
measure cell proliferation
measure cell death
measure cell envelope
measure particle surface
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