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Resazurin, a soluble dye that is reduced to highly fluorescent resorufin in proportion to the metabolic activity of a cell population, is a valuable, noninvasive tool to measure cell number.
For therapeutic antibodies that inhibit the growth of cancer cells, proliferation assays that measure cell number changes after the antibody treatment are often used to determine the potency of the antibody.
We used flow cytometry to measure cell number and individual cells' fluorescence.
At different times after tumour implantation, aliquots of the ascitis fluid were recovered using a 21-gauge needle to measure cell number and mediator/cytokine levels.
Here we present FijiWings as a tool to precisely measure cell number (represented as trichome count) and tissue size (in pixels) in the Drosophila wing using several integrated functions.
To measure cell number in individual crypts, first the length of individual crypts was measured using 3D images of wild-type or APC Min/+ mice intestines stained with DAPI and phalloidin (Appleton et al., 2009).
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The same procedure was used to measure cell numbers in the subventricular zone and in the olfactory bulb.
Hexosaminidase is a lysosomal enzyme catalyzing the degradation of glycosylated cellular constituents and due to its widespread occurrence in cells of different origins, hexosaminidase has been extensively used to measure cell numbers [28].
The colorimetric MTs (Promega) assay was used to measure cell numbers at 24 and 48 h.
In addition, for MCF-7 cells we verified the utility of this assay for measuring cell number by comparison with DNA content measurements and with cell counts by hemocytometer (data not shown).
Cells were treated with nine concentrations of drug for 72 h before measuring cell number relative to controls [12].
More suggestions(15)
measure cell survival
measure cell adhesion
measure cell contraction
measure cell invasion
measure cell migration
measure cell line
measure cell apoptosis
measure cell staining
measure cell cycle
measure average number
measure cell motility
measure cell concentration
measure cell proliferation
measure cell death
measure cell envelope
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