Four histomorphometric parameters were measured: bone volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.S).
For quantitative histomorphometric analyses, we measured bone volume per tissue volume (BV/TV) and osteoblast surface per bone surface (Ob.S/BS) of rabbit bones in MM-bearing SCID-rab mice as the indices of bone destruction and osteoblast function, respectively.
We measured bone volume at the medial femoral condyle and osteophyte volume and determined the OA score and expression of matrix proteins.
However, at the tibia, Hispanic women had significantly lower directly measured bone volume fraction, rod BV/TV, plate and rod trabecular number, and lower trabecular plate and rod connectivity including P P, P R and R R junction densities, but significantly higher rod trabecular length (rTb.ℓ) than Caucasian women.
Additional measures of bone formation were not attempted, as our previous studies have found a high degree of correlation between micro-CT measured bone volumes and bone areas measured by histomorphometry.
A similar method was used to measure patella bone volume [ 19].
The primary outcome measure was bone volume fraction within the defect (BV/TV), determined with high-resolution quantitative computed tomography (HR-QCT) after 5 weeks.
A semi-automatic image analysis system (Image-Pro Plus) was used to measure the bone volume fraction, the trabecular number (#/mm), the trabecular thickness (μm), and the trabecular separation (μm) [ 20].
Traditionally, trabecular microarchitecture is assessed from bone biopsies by two-dimensional histomorphometry [ 116], applying stereologic principals to measure trabecular bone volume fraction (the ratio of mineralized bone volume to total volume [BV/TV]), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), and trabecular number (Tb.N).
Trabecular morphometry was characterized by measuring the bone volume fraction (bone volume / total volume, BV/TV), trabecular thickness (Tb. Th) and trabecular number (Tb. N).
