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The K472E/R473E mutant Cul1 was then used to measure binding of CAND1 by coimmunoprecipitation.
Isothermal titration calorimetry (ITC) was used to measure binding of the individual human or mouse NOXO1 SH3andnd SH3B domains to the p22phox cytoplasmic domain.
A sandwich ELISA was used to measure binding of peptide-free or peptide-loaded DR1 to LB3.1 and MEM264 as previously described [22].
The main technique used for these studies was change in spectroscopic absorbance, which is routinely used to measure binding of molecules.
Figure 1 is a schematic representation of the production of the 32P-labeled peptides and the experimental design of assays to measure binding of peptides to cell lines.
To measure binding of soluble PDGF receptor α to PDGF, recombinant human PDGF receptor α extracellular domain (PDGF sRα) was biotinylated using biotin-X-NHS (Research Organics, Cleveland, OH).
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To determine whether cells that are relatively insensitive to AprA have an observable defect in the ability of rAprA to bind to this receptor, we measured binding of rAprA to cells.
Online substrate cleavage was monitored by measuring binding of mAb10F12 to a SNAP-25 neo-epitope.
Joshi et al. [5] tried to address this problem by measuring binding of phospholipidosis-inducing drugs to L-α-dipalmitoyl phosphatidylcholine vesicles.
We measured binding of CquiOBP1 to a few compounds known/inferred to be mosquito attractants.
We measured induction of Nrf2 activity in whole lung nuclear protein extracts from WT, p47phox−/− and gp91phox−/ mice using an ELISA-based method that measures binding of Nrf2 to its oligonucleotide target.
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