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To directly test whether the proteasome was activated by TNFα, a cell-based assay was used to measure activity of the proteasome [ 15].
In-gel activity assays (Zymograms) were done using carboxymethyl-cellulose (CMC) to measure activity of endoglucanase (i) and esculin for β-glucosidase (ii).
Calorimetry is a generic way to measure activity of enzymes, but unlike conventional calorimeters, chip-based calorimeters can be easily automated and implemented in high-throughput screening platforms.
When p NPG was used as substrate to measure activity of β-glucosidases, the activity reached the highest level of 3.31 ± 0.14 U/ml with use of corn stover + wheat bran as carbon source.
Being able to measure activity of proteins as an entire pathway responds to a target drug, disease state, or other stimulus is far more powerful than genomics, or any other technique invented before proteomics.
We have developed an assay to measure activity of PADs using a RA autoantigen fibrinogen.
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Reporter gene assays measure activities of intact cells as a result of receptor-binding, for example mRNA, protein.
The Barthel Index (BI) [ 22] will be used to measure Activities of Daily Living (ADL).
The Physical Self-Maintenance Scale (PSMS) [ 35] will be used to measure activities of daily living.
In humans, it is important to measure activities of specific enzymes in vivo.
The NDI was originally designed to measure activities of daily living (ADL) in patients with neck pain [ 1].
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