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Because the microbe expresses GFP constitutively, it allows the use of fluorescence as a measure of sugar concentration when the solution is too turbid to measure absorbance accurately or when other compounds interfere with this culture density measurement.
At indicated time, reaction mixtures were withdrawn to measure absorbance at 420 nm.
Remove 3 mL solution from erlenmeyer flasks to measure absorbance at 647 and 664 nm using a spectrophotometer as the amount of extracted chlorophylls every 10 min.
UV Vis spectra of the solutions were obtained using Junway 6100 spectrophotometer to measure absorbance over a range of 200 1000 nm semi-quantitatively.
A dedicated data acquisition system is designed and fabricated using a LPC2106 microcontroller with light emitting diode (LED) as source and photodiode as sensor to measure absorbance and to calculate the calcium concentration.
The reaction between residual ethanolamine and TNBS in borate buffer at pH 9.2 continues for 15 min at 55°C followed by cooling with tap water to measure absorbance at 420 nm.
Similar(29)
Enzymatic activity was quantified by measuring absorbance at 401 nm.
The 800 nm wavelength is optimal for measuring absorbance between emulsified asphalt and cement.
Adenine concentrations were determined by measuring absorbance at 260 nm from filtered samples.
The concentration of aniline, remained in solution, was determined spectrophotometrically by measuring absorbance at 232 nm.
ADA and ADP were detected by measuring absorbance at 485 nm.
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