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The traction force dynamics were even clearer when we quantified them through the contractile moment [24]: at the earliest measurable time point following stretch, the contractile moment was reduced by 70% of its baseline value.
The mean residence time until the last measurable time point, MRTlast was 3.25 h (sd = 0.85 h).
Individual areas under the concentration time curves (AUCs) from time 0 to the last measurable time point (AUC0– t ) were calculated according to the trapezoidal rule.
The mean area under the concentration-time curve until the last measurable time point, AUClast, was 4.205 μg.h/mL (sd = 1.534 μg.h/mL).h/mL
In milk, after administration on D0, the mean observed Cmax was 0.9974 μg/mL and the mean area under the concentration-time curve until the last measurable time point, AUClast, was 4.205 μg.h/mL.h/mL
For the SRD (day 1 for each period), FE and MRD (day 1 only) studies, AUC 0, t) and AUC 0,∞) were calculated, where AUC 0, t) is the area under the plasma concentration time curve to the last measurable time point and AUC 0,∞) is the area under the plasma concentration time curve extrapolated to infinity.
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Finally, hyaluronan was not observed in a measurable quantity at any time point.
Only one patient had evidence of in vitro neutralising antibodies against uricase activity; this was measurable at only one time point.
On the other hand, invasion was measurable at a later time point (20 h) for MDA-MB-231, and the two other model cell lines, MCF-7 and MDA-MB-468.
Analysis included samples in which RAGE and HPRT were both measurable at both time points allowing paired measurements.
Yet, at this time point, no measurable neutralizing antibody titer could be detected, and therefore another boost was given.
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