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These findings are in agreement with the observed disappearance of intact prodrug and concomitant appearance of the corresponding phosphoramidate intermediate derivative in CEM cell extracts without measurable formation of araU monophosphate.
The reaction of the distal histidine mutants with hydrogen peroxide showed no measurable formation of ferryl haem iron, with the main optical changes indicative of haem degradation from radical damage.
Indeed, the substrate with the natural substitution pattern at the isoquinoline part and the missing methoxy in 4′ (1 f) led also to very low, but measurable formation of regioisomer 3 f with a ratio of 2 f/ 3 f 97 3 (Table 1 entry 6).
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The LVP zones had no measurable fold formation until day 27.
Small but measurable water formation is observed over both the catalysts after light-off, which suggests that the RWGS takes place over both the catalysts up to ~700 °C.
A study in Berlin, Germany, documented measurable sulfate formation within 50 km of the source (Lammel et al. 2005).
Measurable particle formation and growth occurred almost exclusively in the 0.1-0.2 0.1-0.2and 0.2-0.3 microm size frandions in all of the experiments.
Sixty minutes after trauma, four out of five animals had no measurable clot formation (EXTEM CFT ≥4,000 s), and clot strength (EXTEM MCF) had reduced to 11 ± 7 mm.
Sixty minutes after trauma, four out of five animals had no measurable clot formation (EXTEM CFT ≥4,000 seconds) and clot strength (EXTEM MCF) had reduced to 11 ± 7 mm.
Because of this, the data generated with UGT1A1 cannot be used to estimate Km values because of a lack of measurable product formation at substrate concentrations below 5 μM.
Despite demonstrating no measurable amine product formation from any of the tested substrates, a broad peak was detected in the tryptophan reaction mixtures for each enzyme.
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