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While CasKi cells expressed both E6 and E7 antigens (Fig. 1a, b), SiHa and HeLa S3 cell lines had no measurable expression of E6 antigen (results not shown) but did express E7 protein (Fig. 1d, e); albeit, the level of E7 expression was low in SiHa cells (Fig. 1d).
At this dose vector targeting also resulted in measurable expression of human TNFα.
Lastly, the basal phenotype exhibits only little, but still measurable expression of curli.
To further confirm E2-induced alternative splicing of above genes leads to measurable expression of distinct protein species, we performed western blotting.
This is likely due to the greater dilution used with PAXgene tubes and the loss of measurable expression of less abundant miRNAs.
The results presented here show that thapsigargin induces significant measurable expression of acALY18 within 60 min and detectable levels of GRP78 within 24 h.
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Establishing conservation targets (also called indicators or endpoints), the measurable expressions of desired resource conditions, can help with site-specific up to landscape-scale conservation planning.
By contrast, only 2 out of the 15 immature gametocyte clusters showed measurable expression in any of the patients, supporting the idea that immature gametocytes are not present in the blood circulation.
Expression analysis of the Malawi patient cohort samples [ 26] indicated that seven out of nine mature gametocyte clusters showed measurable expression within a subset of patients with mature gametocytes as detected by microscopy.
In order to include in clustering analysis only well measured transcripts, we selected spots with a present call (identified transcripts with measurable expression) in at least 80% of experiments and being > 1.7 fold up- or down regulated in at least 5 experiments.
FRα exhibits limited normal tissue distribution, with measurable expression restricted to the apical surfaces of a few epithelia, predominantly in the lung, kidney, and choroid plexus, but is overexpressed in a spectrum of solid tumors, including ovarian cancer, non small cell lung cancer, breast cancer, kidney cancer and in high-grade osteosarcoma [7] [10].
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