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In order to include in clustering analysis only well measured transcripts, we selected spots with a present call (identified transcripts with measurable expression) in at least 80% of experiments and being > 1.7 fold up- or down regulated in at least 5 experiments.
By contrast, only 2 out of the 15 immature gametocyte clusters showed measurable expression in any of the patients, supporting the idea that immature gametocytes are not present in the blood circulation.
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The predicted interactions for targets with a GFP fluorescence signal above background (indicating measurable expression) are shown in Figure 2. Two of the six tested target candidates are translationally repressed by Yfr1, as shown by a reduced GFP fluorescence signal (Fig. 3).
At this dose vector targeting also resulted in measurable expression of human TNFα.
However, in all the mt-p53 harbouring cell lines, the levels of the protein was higher than in the TC252 (wt-p53) cell line, which was only visualised after longer exposure, and there was no measurable expression of full-length p53 in the p53-null (A673) and p53-truncated (SK-N-MC) cell lines.
This mutation is incredibly common in colon cancer (21%), resulting in a premature stop codon and loss of measurable HDAC2 expression in 83% of mutant tumors (Ropero et al., 2006).
Control LV/enGFP injection led to measurable reporter gene expression in hearts.
Triple-primer PCR assay applied to the detection of ERC4 messenger RNA in 18 matched normal and tumor breast tissues gave a measurable value of expression in 36 out of the 36 samples studied.
Consequently, the small handling differences do not seem to have evoked measurable changes in gene expression in this experiment.
The partial knockdown of s-vLN PDF using R6-Gal4 resulted in a significant reduction in morning anticipation without measurable effects on PDF expression in the l-vLNs.
No genes were found to be significantly differentially expressed after treatment of BMDM with hBD3 and analysis using the GOrilla method failed to identify any broader enrichment trends among the most altered genes, demonstrating that hBD3 has little measurable effect on gene expression in the absence of LPS.
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