Differences among group means were analyzed using one-way analysis of variance (ANOVA).
When NU treatment was P < 0.1, means were analyzed with linear and quadratic polynomial contrasts.
Statistical differences between means were analyzed using Duncan's multiple range test after one-way analysis of variance (ANOVA).
Differences among means were analyzed by one-way analysis of variance (one-way ANOVA).
Differences between means were analyzed using the unpaired Student's t-test (two-tailed).
Differences between means were analyzed using a two-tailed Student t test while difference among means were assessed ANOVA.
Differences among means were analyzed by one-way ANOVA followed by Tukey post hoc test for pairwise comparison unless otherwise stated.
Two-fold regulated genes (≥2 or ≤0.5, calculated as the ratio of the means) were analyzed using Ingenuity Pathways Analysis (Ingenuity Systems, www.ingenuity.com).com
Differences in the means were analyzed using two-tailed student's t test or Mann-Whitney test when data did not fit a Gaussian distribution.
Group means were analyzed for overall statistical significance by unpaired Student's t test or one-way analysis of variance (ANOVA) followed by Bonferroni's multiple comparison tests.
For all exposure assays, differences in group means were analyzed for each mosquito population separately, using mortality proportions that were arcsine √ transformed prior to analysis, and compared using a one-way ANOVA SPSSS 16.0) and a Tukey post-hoc test.
