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Analysis of the means of transcript abundance among these seven different species therefore provides an estimate of the variation in gene expression that has evolved among species.
We find that Dcp2 is frequently not involved in pre-mRNA degradation upon SSA treatment implying the existence of other means of transcript decay.
For each gene with multiple transcript variants, we collapsed these parameters into one by choosing the splicing form with the longest protein and calculating the means of transcript isoforms.
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Because IGR probe sets on our custom Affymetrix GeneChip are a low-resolution means of delineating transcript structure, we examined TSS and rpoE2 dependence for putative sRNAs, newly identified ORFs, and 5′-UTR detected by these IGR probe sets.
All candidates showed support for the single predicted isoform by means of available transcript evidence and consequently only this single isoform could be detected during experimental validation (Additional file 2).
for j=1,…,d, where the parameters (mu _{j}^{0}) and (mu _{j}^{1}) specify the mean abundance of transcript j in the inactivated and activated states, respectively, and {v k ;k=1,2,…} is the measurement noise process, with a standard AR(1) structure {mathbf{v}}_{k},=,eta,{mathbf{v}}_{k-1}+ 1-eta),mathbf{w}_{k-1}+ 1-eta
In most T2 lines (9 out of 11) the mean level of transcript was lower than in the parent T1 plant.
(C ) Mean values of transcript levels from all four experiments were clustered by hierarchical using the HCL module of MEV program with Pearson correlation and the average linkage method.
Zap1 mediates ADH1 and ADH3 repression in low zinc by means of intergenic transcripts that are activated by Zap1 and transcribed through the ADH1 and ADH3 promoters.
For example, running Velvet on the rice data (see Section 3.2 for details) results in contigs of mean length 245 bp only, while the mean length of transcripts is about 1700 bp.
Traveling ratios (TR) were calculated from the mean of summed transcript RPM means for each transcription group in an area representing the promoter (p) (−0.3/0.3 kbp) and downstream body (b) (0.3/2.25 kbp) of the transcript, TR = (b/p).
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