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This can be done by means of substrate resources descriptors.
Insights into binding can guide computational docking approaches that test the fit of different structures onto kinases as a new means of substrate finding.
In C. crescentus, TonB-dependent receptors appear to be the dominant means of substrate transport across the outer membrane and the C. crescentus genome codes for an extraordinary sixty-seven TonB-dependent receptors, leading to the suggestion that a repertoire of diverse active transport pathways can be established in the outer membrane of this bacterium [37], [38].
It is also the case that diffusion may not always be the main means of substrate transport to the compartment and that the reaction maybe more complex than the simple Michaelis-Menten type reaction of these previous studies.
This region provides an extension of the protein surface next to the metal-binding site (Fig. 2; also observed for HjGH61B), putatively providing means of substrate binding as this region also contains exposed aromatic amino acids that are known to interact with carbohydrates.
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We estimated microbial biomass by means of substrate-induced respiration, microbial activity as basal respiration (BR) and as a kinetically derived parameter (r) hypothesised to correspond to the active fraction of the microbial biomass.
For each reaction, take the harmonic mean of substrate concentrations (zero/v. low/low/med/high/v. high); then reaction rate is (zero/v. low/low/med/high/v. high).
The substrate recognition of Pseudomonas cepacia lipase in the acylation of nucleosides was investigated by means of rational substrate engineering for the first time.
The gelatinolytic activity of matrix metalloproteinases-2 (MMP-2) secreted in conditioned media was assayed by means of gelatin substrate gel electrophoresis.
Immune complexes were detected by means of TMB substrate for 15 min and quantified by measuring the absorbance at 405 nm and 490 nm.
However, evidence of both pathways has been found in S. schenckii by means of specific substrate supplementation or drug-related inhibition of the respective pathways [ 22, 23, 103, 104].
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