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The low concentration of Paclitaxel in the original T. brevifolia and the supply crisis of Taxol or Taxotere stirred the interest to produce these compounds by means of isolation from all other 10 geographically isolated Taxus species including T. canadensis, T. cuspidate, T. mairei, T. baccata, T. wallichiana, and T. yunnanensis.
The Kurds, part of the world's largest stateless minority, have been heading in that direction since 1991, when Saddam Hussein started to punish them by means of isolation and sanctions.
Despite the acknowledged necessity to assess the variability of S. cerevisiae populations, the identification of their composition has been so far mainly carried out by means of isolation, a process requiring time and specialized operators9,12,13,14,15.
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To investigate the spatial genetic structure of mountain gorillas, we analysed the genotypes of 193 mature individuals at 11 microsatellite loci by means of isolation-by-distance and spatial autocorrelation analyses.
Yet, by means of his isolation and his stoicism, he becomes almost a romantic figure.
This assay evaluates the amount of topoisomerase II stably associated with DNA (i.e., identifies the generation of stable cleavage complexes) by means of DNA isolation followed by immunoblot for detection of associated enzyme.
Effective means of overcoming isolation are urgently required to meet the needs of trainees and the health system.
We remark parenthetically that remoteness of a refuge cannot necessarily substitute for a cage as a means of refuge isolation.
Referred to as rocking isolation, allowing for foundation uplift has been proposed, and in a few exceptional cases employed in practice, as a means of seismic isolation 15 – 15.
Our finding of a very restricted intracellular interaction of flotillin-1 and caveolin-1 may also help to explain why by means of membrane isolation or conventional LSM technique, interactions of the two proteins were not reported [ 12, 13].
They are expected to be maintained by different means of reproductive isolation that prevent the minority cytotype from losing too many gametes in crosses with the majority cytotype, which eventually would lead to its extirpation (minority cytotype exclusion principle; Levin, 1975; Husband, 2000).
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