Sentence examples for means of affinity from inspiring English sources
His-tagged human Tau and His-tagged human GSK3 β were expressed in E. coli BL21 and purified by means of affinity chromatography.
The recombinant polyhistidine-tagged FKBP12.6 fusion protein was obtained from the supernatant of the cell homogenate by means of affinity chromatography columns (Protino Ni-Ted kit, Macherey-Nagel, Düren, Germany).
We made use of this sequence to make the β2-actinin peptide and confirmed that it is used for α-actinin-1 binding by means of affinity chromatography and also FRET analysis, showing that the peptide interferes with the signal between LFA-1 and α-actinin-1.
The thick band was separated on SDS-PAGE (Figure 1B, lane-3) by means of the affinity chromatography with Ni2+-NTA resin.
Compared to previous low resolution NMR studies, the high-resolution crystal structures reveal an enlarged lexicon of peptide recognition by the MLLE domain of PABPC1 and provide an example of binding site duplication as a means of enhancing affinity.
To further explore MVI function in myogenic cells, we performed a search for its binding partners by means of an affinity chromatography with the GST-tagged globular tail domain of MVI (GST-MVI-GT) used as a bait.
These small molecules solubilize host ferric iron for import as a bacterial nutrient source and transport it back to the bacteria by means of high affinity transporters (see review, ref (1)).
Dilthey writes "we understand individuals by means of their affinities, their commonalities.
EBFP2, EGFP, and tdTomato were expressed in E. coli BL21 (DE3) and purified by means of Co2+-affinity chromatography.
After four hours of incubation, the cells were harvested and the recombinant EspB protein, which contains a His-tag, was purified by means of Ni2+-affinity chromatography (Xpress purification kit, Invitrogen).
By means of CXXC-affinity purification followed by deep sequencing (CAP-seq), and genome-wide bisulfite sequencing (BS-seq), we designated body-methylated and unmethylated genes in each tissue.
