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Peripheral blood monocytes were isolated from mononuclear fraction by means of adherence and cultured in RPMI1640 as described previously.
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The mean of adherence measured by MEMS was 74.9% (range 53.4% to 92.9%), compared to 84.0% by the self-report questionnaires (range 68.35% to 95.0%).
We did not observe significant differences in the mean of adherence to the MedDiet depending on the duration of diabetes (P=0.227), indicating that the pattern of adherence to the MedDiet remains stable regardless of the diabetes diagnostic.
For the objective measures, although PR has been shown to be a highly effective means of monitoring adherence in adults [ 18], in our study PR overestimated adherence when compared to MEMS.
Directly observed therapy (DOT) is used in the treatment of tuberculosis to enhance the likelihood of treatment success and minimize the emergence of drug-resistant microbes, and has been proposed as a means of improving adherence to HAART [18], [19].
As low adherence to anti-retroviral therapies is significantly associated with mortality and increased risk of mother-to-child transmission [16], [17], it is important to establish other means of improving adherence in this high-risk group in order to improve clinical outcomes in both mothers and their infants.
Most importantly, identifying systematic means of improving adherence among vulnerable populations is unique.
That study estimated the average cost of different ways of directly observing tuberculosis treatment as a means of improving adherence.
Motivational interviewing, guided by suggested clinical questions, is a practical means of strengthening adherence to diabetes self-management.
Education and reinforcement should be evaluated as a means of improving adherence to interventions proven to be effective.
The measurement of serum levels and the tissue concentration of these drugs are a better means of assessing adherence than self-reporting.
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