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We have demonstrated that the GSH capping is a valuable means for subsequent protein crosslinking.
To obtain means for subsequent analysis, 0 30 min data were averaged to obtain steady-state baseline values, and for comparisons against baseline, 40 90 min data were averaged to obtain steady-state infusion period values, and 100 120 min data were averaged to obtain steady-state recovery period values.
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For analysis, all 6 values were averaged, with mean used for subsequent statistical analysis performed with GraphPad Prism statistical software using two-tailed Student's t-tests.
Therefore, we chose mean temperature for subsequent analyses.
For data analysis, as a preliminary step, the first and second radiographic readings of lesion depth at baseline and the two readings at 6 months were examined to determine the validity of using the mean values for subsequent statistical analyses.
Each sample was measured in duplicate and the mean was used for subsequent calculations.
All data from the individual donors was run in triplicate and the mean was used for subsequent analysis.
This procedure left three replicates within the set of which the mean was used for subsequent analyses.
The frequency distribution of echo intensities (Gray units) for the whole image was then calculated and the mean value retained for subsequent data analysis.
In addition, mean fatigue values for subsequent cycles were numerically better than (and for most comparisons, also statistically different from) the value in the first cycle (Table 1).
The mean total cost for subsequent years was £79,000 (SD = £72,000) in intervention sites and £93,000 (SD = £95,000) in control sites.
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