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Primers used are listed in Additional file 11 where forward primers were designed to span the exon borders (for all except L23 and AtWRI1 for which it was not technically possible or meaningful, respectively) to minimize amplification of genomic DNA.
Sesboüé et al (2012) site four studies to support their claim that only 'true' EGFR amplification would be meaningful for the treatment response.
Meaningful differences in the level of the amplification signal measured are considered those where ΔCt = >|1.7|.
Further attempts to evaluate meaningful predictive markers for EGFR-blocking agents in colorectal cancer focused primarily on gene amplifications and polymorphisms of the EGFR gene.
Information on human epidermal growth factor receptor 2 (HER2) amplification, stage at time of diagnosis and treatment was too frequently missing for a meaningful data analysis.
The subset of patients harbouring KRAS amplifications in FISH analyses with available microarray data was to small to allow meaningful statistical analysis (n=3).
For example, sequence quality evaluated across benchtop systems did not consider library preparation [ 7], and recommendations of amplification-free protocols that require >2 μg of DNA to minimize biases [ 8] are not meaningful for DNA-limited applications.
Meaningful job.
— but meaningful.
Meaningful consultation.
OF AMPLIFICATION about asbestos.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com