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At 120 days after placement, mean viability of C. parvum oocysts was 10% although at a few sampling points, 30% of oocysts were still potentially infective; whereas 90% of A. suum eggs were viable at all sampling points.
The mean viability of STAT1H clones was 16.02±6.7%, while that of STAT1L clones was 1.66±6.21% (Student's two-tailed t-test; p = 0.00001; Figure 5A; Table S2).
In the case of mononuclear AML cells similar trends were observed, the mean viability of willow extract treated cells was 26.2% when compared to the control (96.9%; Figure 2).
The corrected viability for cells transfected with each siRNA was calculated as a percentage of the mean viability of the control wells containing GFP or scrambled siRNAs (Qiagen DNA repair subset v2.0) for each 96-well plate.
Each data point (10,000 cells) represents the mean viability of three independent experiments ± SEM.
Next, cells (mean viability of cell populations: 75 90%) were resuspended in PBS + 5% FCS at a concentration of 100 × 106 cells/ml for intraspinal cell transplantation.
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Comparison of the mean viability values of three replicates for each shRNA from the two individual screens revealed high reproducibility (r = 0.89, Pearson's correlation coefficient).
Here, Σ ^ is the sample covariance of errors, μ ^ i is the vector of mean viabilities for genotype i, and ES is the effect size.
The total mean cell numbers per gram of tissue and their mean viability calculated for each of the tested protocols for the isolation of gut leucocytes are shown in Table 1.
Figure 2 shows the mean viability (95% confidence intervals) of E. coli after 12 h of sonication.
To illustrate the shape of the trajectories we fitted two-segment regressions of the mean viability among replicates onto generation using the stats and segmented packages in R (Muggeo, 2009): (lm viability∼generation); segmented.lm obj=lm_viability, seg.Z=∼generation, psi=G40)).
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