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Similarly, mean vessel length was also reduced by 50% following co-administration of DEX with docetaxel (P<0.05).
Mean vessel length and area, as well as total vessel length and area in 15 fields in each of the triplicate wells were calculated (Microsoft Excel software).
Administration of DEX alone had a minimal but not statistically significant effect upon either mean vessel area or mean vessel length.
However, addition of DEX did potentiate the pronounced antiangiogenic activity of docetaxel, reducing both the observed mean vessel length and mean vessel area.
Docetaxel, in contrast, had a marked effect on vessel development resulting in an almost 90% reduction in mean vessel area (P<0.05) and an over 70% reduction in mean vessel length compared with untreated cells (P<0.05).
There was a significant difference between those spheroids which had been infiltrated with human macrophages, for all parameters measured on day 3; increased vessel number P<0.011, increased number of junctions P<0.025 and shorter mean vessel length P<0.046 all indicating increased angiogenesis in the presence of macrophages.
Similar(54)
Changes in the development of blood vessels after nanoparticle treatments were observed by measuring changes in the mean vessel area, vessel length and the number of branch points.
To determine mean vessel density, the sum length of IB4+ cerebral vessels was determined from a single 20× field.
No difference was observed in the remaining factors (vessel length and mean radius).
This corresponded to a mean number of rolling leukocytes per unit vessel length of 2.00/mm (SD 1.67) and 0.75/mm (SD 0.55), respectively (P < 0.001).
This corresponded to a mean number of adhered leukocytes per unit vessel length of 0.16/mm (SD 0.22) and 0.03/mm (SD 0.06) for sepsis and non-infected groups, respectively (P < 0.001).
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