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The mean vesicle size of the nanoliposomes was measured by a laser scattering method (Nano ZS 90, Malvern, UK).
The prepared formulations were characterized in vitro for vesicle morphology, mean vesicle size, and percent drug entrapment.
The mean vesicle size of optimized liposomes ranged from 90 to 100 nm with low polydispersity index (< 0.5).
B Mean vesicle size with standard deviation.
The difference in mean vesicle size at neuromuscular and neurociliary synapses in motorneurons also supports a mixed neurotransmitter identity.
The physical stability was evaluated by mean vesicle size, EE%, and zeta potential measurement over a three-month period.
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The average vesicle size was 951.02 nm with a zeta potential of − 51.87 mV.
NanoSight™ tracking analysis (NTA) software version 2.3 was used to produce the mean and median vesicle size together with an estimate of particle concentration.
The mean liposome vesicle size ranged between 89 and 100 nm across all four formulations through the 2-year time point (Table I).
We thus measured the mean pool size of neighbours around the individual synapses and found that an increasing number of neighbours correlated with a larger mean vesicle pool sizes of the surrounding synapses (Cohens d = 2.30; Spearman's ρ = 0.95, p<0.001).
The mean and standard deviation (SD) of the vesicle size distribution were recorded using the following instrument parameters: temperature, 23°C; viscosity, 0.933; refractive index, 1.333; intensity set point, 300 KHz; channel width, 10 μs; scattering angle, 90°; run time, 5 min; mode, vesicle; and Gaussian distribution.
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