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The mean vesicle fusion time given by eq. 16 depends on the SNARE density and patch velocity.
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(A ) Schematics of Models 1 and 2. (B and C ) Comparing the Fano factors (C ) generated by one-step (Model 1) vs vesicle fusion based peroxisome biogenesis (Model 2) at equivalent mean peroxisome abundances (B ).
DOI: http://dx.doi.org/10.7554/eLife.04553.002 Precise control of synaptic vesicle fusion at the presynaptic terminal endows a nervous system with the means to regulate functional synaptic connectivity.
At any given terminal, vesicle fusion can occur hundreds of times per second, as trains of impulses arrive one after the other.
They give the terminal the capacity to recycle just 10% of its entire vesicle pool, so it seems that recycling takes place slowly, in the seconds following vesicle fusion, and so does not contribute when multiple vesicles fuse in quick succession.
The two stages of cluster fusion, a fast early and a slower later stage, were detected also in vesicle fusion.
At high peptide/lipid ratio, V4 can permeabilize vesicles composed of negatively charged lipids and eventually cause vesicle fusion.
The thinning of the hemifusion diaphragm during its formation results in the opening of a fusion pore for vesicle fusion.
Some patients have antibodies that bind synaptotagmin, the protein sensor for calcium-regulated vesicle fusion.
Thus MV2 vesicle fusion apparently does not proceed unless adjacent to previously fused vesicles.
Typical measured fusion times greatly exceed the ∼1 ms required for synaptic vesicle fusion [15].
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