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A Mean vesicle count with standard deviation.
This number was always close to 0 because we adjusted all measured counts by subtracting the mean vesicle count at time t = 0 and considered the adjusted measurements in fitting.
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The mean vessel count obtained from five fields at × 200 magnification was used to define the mean vesicle density (MVD).
The prepared formulations were characterized in vitro for vesicle morphology, mean vesicle size, and percent drug entrapment.
The mean vesicle size of the nanoliposomes was measured by a laser scattering method (Nano ZS 90, Malvern, UK).
The 'mean vesicle count per cell' was determined from all nuclei captured in 3 images/well, and from 8 or more replicate wells per cell line.
The Transfluor module of MetaXpress software (Molecular Devices) was used to determine the 'mean vesicle count per cell' from captured images.
For all conditions and vesicles types, we calculated vesicle count and vesicle size.
B Mean vesicle size with standard deviation.
The addition of BAF increased the vesicle size as well as the vesicle count of lysosomes, most notably for autolysosomes.
As expected, under FM conditions the model predicted a near steady-state vesicle count and vesicle size.
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