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CD34 highlighted the marked differences in the vascularity of the two components with mean vascular count of 3 vessels per 200 tumor cells in the meningioma component and 18 vessels per 200 tumor cells in the osteosarcoma component.
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The number of tumour-infiltrating vessels per 0.5 mm was assessed as mean of vascular counts in five fields of view adjusted to 0.5 mm in vital tumour tissue.
The vascular count (mean number of vessels per 0.5 mm) in vital tumour mass of melanomas treated with ML-I at a concentration of 500 ng kg−1 was significantly increased compared to the control group (42.0±1.7 and 32.4±1.6, respectively; P=0.001) and to the 30 and 150 ng kg−1 group (34.74±1.3 and 35.1±1.6, respectively, P<0.05; Supplementary Information 2c).
The a-MVD is determined using the same grid and magnification (200×) as for h-MVD and calculating the mean of the vascular counts obtained in at least 10 15 random fields for each tissue section.
Mean vascular density was quantitated by counting the number of CD 31-immunostained blood vessels and by morphometric assessment of stained vessel area by computer-assisted image analysis.
The mean vascular area percentage and the mean manual vessel count data have been calculated with the MS Excel software.
The mean vascular diameter is also changed after treatment.
Vascular count, VEGF or TP expressions were not significant in either analysis.
In this analysis, vascular count failed to show any independent influence on overall survival (OS).
The mean microvessel count of the five most vascular areas was taken as the MVD, which was expressed as the absolute number of microvessels per 0.74 mm2 (×200 field).
The mean microvessel count of the five most vascular areas was taken as the MVD, which was expressed as the absolute number of microvessels per 0.74 mm2 (×200 field) on an Olympus microscope (CX-31) with an Olympus camera C-5050ZZ).
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