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For each replicated experiment within a dataset, the mean transcript value per experiment was calculated.
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To dichotomize the 54 tumor samples according to levels of expression for each of the NFκB and AP-1 upregulated genes identified in the significant probe set, samples with positive mean-centered transcript values were designated as "high" expressors while those with negative mean-centered values were classified as "low" expressors.
Normalization of gene transcript abundance values was performed by dividing each transcript abundance value by the mean transcript of this gene across all samples and then taken the logarithm with 2 as the base.
Bars represent the log2 values of the ratio of the mean transcript levels for inoculated vs. non-inoculated plants from two technical replicates.
We identified non-additive transcripts as those showing significant deviation of mean transcript levels in hybrid plants from combined mean transcript accumulation of parental accessions (e.g. those hybrid transcript values falling outside the 99% confidence interval of the linear model associating hybrid transcript with mean parent transcript levels).
To plot mean transcript abundance against CpGO/E, genes were divided into 25 equally sized quantiles based on CpGO/E values.
Positive values mean the Ct value was higher for the trypsin-like serine protease and thus less transcript than β-actin in the sample; the converse is the case if the value was negative.
FastMap allows the user to select strata by genotype a priori, and subtracts strata means or medians from the transcript values in each stratum (Pritchard et al., 2000).
Only 159 contigs had mean hybrid read counts outside the 99% confidence intervals generated for predicted hybrid transcript values in both analyses (Additional file 5: Table S1).
(C ) Mean values of transcript levels from all four experiments were clustered by hierarchical using the HCL module of MEV program with Pearson correlation and the average linkage method.
Transcripts were then categorized in groups on the basis of their relative transcript abundance, as measured by the mean fluorescence value (at 635 nm) of each transcript.
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