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The mean transcript size was 497 bp, with lengths ranging from 201 to 9744 bp.
Similarly, the mean transcript size was ~50% smaller than observed in a random set (t test; P = 2.6e - 9).
Mean transcript size multiplied by number of spider blastx-positive components (genes) – with and without an additional 15% of components based upon Markov-ORF analysis (see text).
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This approach identified transcripts from 4699 genes as present in at least one copy in 100,000 reads; using an average transcript size of 1.3 kb, this means the low detection cut-off was approximately equivalent to an average RPKM of 7.7.
Plus and minus strand were summarized by calculating mean values for all transcript size intervals.
The mean transcript length, maximum transcript length, and N50 size of Trinity transcripts were 1112, 22,887, and 1863, respectively.
We converted the gel images to plots of transcript length by densitometry and comparison to size standards, which allowed calculation of mean transcript lengths for each time point.
The mean transcript length is 564 nucleotides.
(B ) Mean transcript lengths were calculated from two independent experiments.
(B ) Mean transcript lengths were averaged from two independent experiments.
This method allows the determination of approximate transcript sizes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com