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The mean transcript count at all four time points was used.
The Z-score numerical value is calculated by taking for each gene and tissue the (RPKM -normalized log2-tRPKM -normalizedcript count, subtracting the normalized mean transcript count of all tissues and dividing by transcriptrd deviation of the normalized transcount count of all tisubtracting
Calculation of the Z-score was determined based on the RPKM-normalized log2-transformed transcript count data as follows: Z = (X-μ /σ, where X-μ /σhe transcript count of a gene for a specific tissue/timepoint, μ is the mean transcript count of a gene across all tissues/developmental stages and σ is the transcript count standard deviation of a gene across all tissues/developmental stages.
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Linear modeling of mean transcript counts from F1 plants as a function of mean parent transcript levels was performed in R. Examination of residuals and leverage estimates for this model led us to remove 3 contigs with values of Cook's D exceeding 1. Refitting the model without these points did not significantly alter the parameter estimates for the model.
(C) Transcript count with a threshold of negative minimum TPM value.
Right y-axis is the transcript count in that function item, left y-axis is the corresponding percentage of transcripts number Open image in new window Figure 3 Transcript number in each EggNOG function classes.
Left y-axis with bar plot is the transcript count in each bin and right y-axis with point plot is the accumulate count below that bin.
The mean transcript length is 564 nucleotides.
mean activity count per minute.
The mean eosinophil count was 72.4 Eos/HPF.
The mean granulocyte count was 5310 μl−1, the mean platelet count was 15.3 × 10 μl−10
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