Sentence examples for mean transcript accumulation from inspiring English sources

Mean transcript accumulation (RPKM) per accession (A =  H. annuus cmsHA89, H = F1 hybrid, P =  H. petiolaris Pet2152) are provided for both full ("full") and reduced ("red") analyses.

The mean transcript accumulation estimates for these contigs in F1 plants ranged from 1.2 to 2372% of predicted values, roughly evenly divided between those above (44%) or below (56%) the parental mean.

A linear model of mean transcript accumulation across F1 plants as a function of the mean of parental samples explained a high proportion of F1 transcript variance (p < 0.0001, R = 0.98 (reduced data), p < 0.0001, R = 0.96 (full data)).

We identified non-additive transcripts as those showing significant deviation of mean transcript levels in hybrid plants from combined mean transcript accumulation of parental accessions (e.g. those hybrid transcript values falling outside the 99% confidence interval of the linear model associating hybrid transcript with mean parent transcript levels).

The mean log2 transcript accumulation per gene per experimental unit (accession, tissue, stress, or time point) was used for further analysis.

To compare variance across experiments and transcripts, I first estimated the per transcript variance (σ2) across the mean log2 transcript accumulation per experimental unit for each transcribed locus measured within a given experimental dataset (Table S1).

Reference contigs showing mean hybrid transcript accumulation outside the confines of the confidence interval for predictions were classed as "non-additive".

Bootstrapping analyses were employed to test if the mean VMR and CV for transcript accumulation within each metabolic pathway were significantly different from a random genomic sample.

In addition, 50 reference contigs identified as showing non-additive F1 transcript accumulation had F1 mean values either higher (41) or lower (9) than both parent mean values (Additional file 5: Table S1).

This means that our quantification of transcript accumulation was highly reproducible within one sample and that technical error has little impact on the values reported here.

In the figures, transcript accumulation data are plotted as mean ± standard error of the mean.