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The probe sets exhibited significant, greater than 1.5-fold, differences in mean transcript abundance among the described cultures.
To plot mean transcript abundance against CpGO/E, genes were divided into 25 equally sized quantiles based on CpGO/E values.
To investigate broad scale relationships between the magnitude of gene expression and CpGO/E, we plotted mean transcript abundance (across all samples) for each gene against its CpGO/E value.
Mean transcript abundance for more than one-half of the genes in oocytes from patient 6 were higher than those from either patient 4 or patient 5, again reaffirming interpatient differences (Table 2).
A p value was calculated by evaluating the significance of the difference observed in the mean transcript abundance for each probe set between the SRShypo, SRSnormo, and Cnormo groups.
Mean transcript abundance varied significantly across CpGO/E quantiles (ANOVA p < < 0.0001) and genes in the high-CpGO/E component showed decreased mean expression compared genes in the low-CpGO/E component (Welch Two Sample t-test; p < < 0.0001).
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Remaining columns: ratios of geometric mean transcript abundances of MFS affected vs unaffected control for three subject groups, and Wilcoxon p values for the null hypothesis of no between group differences.
The section headings were changed into: Age factor 1 (age related variation in mean transcript abundances) Age-factor 2 (age related variation in residual values) Moreover to improve the understanding of the two age factors figure 8 was added.
Analysis of the means of transcript abundance among these seven different species therefore provides an estimate of the variation in gene expression that has evolved among species.
It should be noted that, while they provide a means to survey transcript abundance on a genome-wide scale, the sensitivity of microarray assays is low compared to other approaches such as real-time PCR.
The genes in the analyzed lists were ordered from the highest to the lowest average deviation from the mean of the relative transcript abundance value calculated across samples.
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