In our study, this index was related to mean trabecular bone area in the OP group, but there was no relation with structural parameters in OA group.
Although the mean trabecular bone area in OA is higher than in OP, there was no statistical significance between them.
The mean cortical bone thickness (CBT) and the mean trabecular bone thickness (TBT) were measured in 5 fields/slide from 5 slides for each rat.
The mean trabecular bone volume constituted 28,4 ± 3,82% with a mean trabecular width of 225,12 ± 16,30 μm.
Afterwards, a three-dimensional (3D) analysis for the indices including bone volume density (bone volume/tissue volume, BV/TV, %), bone surface density (bone surface/tissue volume, BS/TV, mm−1), mean trabecular thickness (Tb.Th, mm), mean trabecular number (Tb.N, mm−1), and mean trabecular separation (Tb.Sp, mm) was done for the spongy bone [ 14].
Trabecular bone volume per total volume (BV/TV), mean trabecular thickness (Tb. Th), mean trabecular number (Tb. N, and mean trabecular separation (Tb. Sp) were calculated using Scanco μCT V6.1 software.
Trabecular morphometric traits were computed from binarized images using direct 3D techniques that do not rely on prior assumptions on underlying structures: trabecular bone volume per total volume (BV/TV), mean trabecular thickness (Tb.Th)., mean trabecular number (Tb.N ., and mean trabecular separation (Tb.Sp).
The following parameters were determined from the trabecular bone using a direct three-dimensional approach [40]: total bone marrow volume including the trabeculae (TV; mm3), trabecular bone volume (BV; mm3), trabecular bone volume fraction (BV/TV), mean trabecular number (Tb.N; 1/mm), mean trabecular thickness (Tb.Th; mm), and mean trabecular separation (Tb.Sp; mm).
Structural parameters included the apparent trabecular bone volume fraction (BV/TV), apparent trabecular number (Tb.N), apparent trabecular separation (Tb.Sp), apparent trabecular thickness (Tb.Th), mean trabecular area, mean trabecular perimeter, mean trabecular roundness and mean skeleton length [ 41].
This would also mean that trabecular bone adaptation could be modeled as a system responding to an input signal at the onset of the process in a predefined manner, without feedback from the outputs.
The mean fractional trabecular bone volume of the ON group was 0.45 (± 0.07) (n = 8) whereas it was 0.51 (± 0.03) in the AMG treatment group (n = 8).
