Sentence examples for mean threshold cycles from inspiring English sources

Mean threshold cycles for the SpPI and G6pdh reactions were calculated for each sample.

Mean threshold cycles were determined from two technical replicates by the comparative Ct method and expression levels were normalized to GAPDH.

We found good reproducibility as indicated by the intra- and inter-assay CV in the mean threshold cycles (Ct) of a pooled quality control sample of 1.2% and 1.9%, respectively.

The amount of DNA was estimated as follows: 2^(Ct reference Ct sample), where Ct reference and Ct sample are mean threshold cycles of RT-qPCR done in duplicate from GAPDH (reference) and the DNA flanking the I-SceI site (sample).

A standard curve of quantitative real-time (q PCR-based mean threshold cycles (CT) from a 96-plex reaction was established by analysing the results of assays on known numbers of cells (1, 2, 3, 4, 5, 10, 15 and 20) from cell lines that are easily counted and loaded.

The percentage of digestion was estimated as follows: 2^(Ct reference Ct sample), where Ct reference and Ct sample are mean threshold cycles of RT-qPCR done in duplicate on DNA samples from GAPDH (reference) and the DNA regions surrounding the I-SceI site (sample).

The mean threshold cycle (Ct, the first cycle at which an exponential growth of PCR product is detected) value of stimulated sample was compared to that of unstimulated control sample using the Ct value of GAPDH as an internal control.

The mean threshold cycle values for the genes of interest were calculated from three experiments.

For all experiments, the amount of target and control gene was determined from the respective standard curve by conversion of the mean threshold cycle values.

Threshold cycle number of duplicate reactions was determined using the Opticon software and levels of selected gene mRNA expression were normalized to hypoxanthine phsophoribosyltransferase (HPRT) levels using the formula 2 Rt Et), where Rt is the mean threshold cycle for the reference gene HPRT and Et is the mean threshold cycle for the experimental gene.

A standard curve with serial dilutions of hESC genomic DNA ranging from 0.78 to 25ng/reaction in 2-fold dilution steps was prepared by plotting mean threshold cycle (Ct) values against log-transformed gDNA concentrations.