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The last column shows the calculated concentrations of respective probes required to achieve mean T wait of 1 s in a real-time nascent RNA detection experiment.
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Mean binding time T wait was estimated by fitting the probability distributions to a single exponential function.
(B ) Fluorescence time trace corresponding to the montage in Panel A. (C ) Probability distribution of F1 probe binding times fit to a single exponential function to determine the mean binding time T wait.
The mean waiting time of PU t wait - pu nonpre is given by t wait-pu nonpre = AQ pu / ( λ p ( 1 − P BL-pu nonpre ) ). (21).
The blocking probability of PU is obtained as P BL - pu pre = P N. The mean waiting time of PU t wait - pu pre = 0, since PUs in the preemptive mechanism have priorities over SUs.
The right subfigure shows that P FT - su pre increases with λs and λp, while P FT - su nonpre stays at zero. Figure 10 shows that there exists mean waiting time of PU t wait - pu nonpre in the NP mechanism, and t wait - pu nonpre increases with both λs and λp.
Fit error for T wait is given for 95% confidence intervals, and error of the mean was calculated as σ/√(Nmolecules).
Thus, the waiting time can be computed by T wait D i = L i k + 1 B D − T (15).
t wait - pu pre stays at zero, while t wait - pu nonpre increases with λs and 1/μs.
Therefore, the waiting time can be obtained by T wait D i = L i 1 B D − T (14).
Hold and wait can be expressed as ((R j,T i )∈E)∧((T i,R l ∈EE)∧((R l,T l )∈E),l≠i,which means T i has held at least one resource R j and requested a new resource R l, but R l has been held by T l.
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