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The higher protrusion density observed in the midazolam group was associated with a significant decrease in mean spine head diameter (0.62±0.03 µm, control vs 0.41±0.01 µm, mida, P<0.001 Fig. 1c).
Mean spine head diameters were significantly reduced following a 5 h midazolam anesthesia at PND 20, and less at PND 30 (mean decrease at PND 15: 35±1.2%; at PND 20: 37±4%; at PND 30: 10±2%; P<0.01, Fig. 1c).
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We found that the neck opening (width of around 150 nm) occupies as little as 1% of the surface area of the spine head (mean diameter of 600 nm).
We found that in the spine neck, the distance between the two structures was generally below 50 nm (24±28 nm, mean ± standard deviation, N = 16); in contrast, in the spine head we observed a larger apparent distance between the cytoskeleton and the membrane (46±40 nm, N = 19).
The plot shows a typical dendritic base, spine neck and head pattern, with the highest signals at the spine head region.
Spine size (measured across nights, 24 hr interval) was measured as the integrated optical density of each spine head, background-subtracted (using the optical density of neuropil next to the dendrite) and normalized to the mean brightness of the adjacent dendritic shaft.
We show that the mean residence time of a Brownian particle, such as an ion or molecule inside the spine, and of a receptor on its membrane, prior to absorption at the dendritic shaft depends strongly on the curvature of the connection of the spine head to the neck and on the neck's length.
Spine head width was defined as the maximum dimension of the spine head, whereas spine length was defined as the distance from the base of the spine neck to the tip of the spine head.
Among these, is the geometry of the spine head.
Six for the spine head and ten for the neck.
The spine head measurements were limited to the thin and mushroom-shaped spines, as the head of stubby spines was difficult to distinguish from the dendrite shaft.
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