And I mean SPECIFIC wants and needs.
ELISA data was normalized by dividing specific antibody secretion rates for each timepoint by the mean specific antibody secretion rate of the parental clone across all timepoints.
P i (mg) is the total amount of secreted product at the time t i and CDM i (g) represents the total CDM at the time t i (1) The mean specific product secretion rate qP, mean (mg g 1 h–1) was calculated with Equation (2), where t E (h) is the time at the end of cultivation.
In P. pastoris, the mean biomass-specific secretion rate (qp) was 40-fold lower for 3D6scFv-Fc compared to HSA.
Results were expressed as δ pg/ml, after deducting secretion in the absence of stimulus to normalize the expression of mitogen/antigen specific secretion.
Taken together, X12345 secretion may be the result of semi-specific secretion.
PtXyl43 secretion is a two-step process, and the second step is accompanied by cell periplasmic leakage, indicating that PtXyl43 secretion is the result of semi-specific secretion.
This may be because the X12345 secretion to the periplasm caused instability of the outer membrane as described in semi-specific secretion (Georgiou and Segatori 2005).
These are substrate-specific secretion machineries that share a similar architecture and secretion mechanism [ 88].
We also defined a sIEC-specific secretion profile based on a thorough literature review.
