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A detectable killing is defined here as a mean specific lysis of more than 5% at least for the two highest effector target ratios.
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The mean percentage of specific lysis of triplicate wells was calculated according to the following formula: Specific lysis = [(Specific release − Spontaneous release)/(Maximal release − Spontaneous release)] × 100.
The treatment of targets with IFN-γ increases their sensitivity to CTLs with 60 ± 9% specific lysis (mean ± standard error, n = 3).
Cytotoxic activity was expressed as the percent specific lysis, calculated by the equation 100 - mean percent survival of peptide-loaded targets.
The mean of triplicate measurements was expressed as a percentage of specific lysis using the formula: (sample release – spontaneous release)/(maximal release – spontaneous release) ×100.
Cytotoxic T lymphocyte (CTL) assays were performed with B-16 targets for 48 hr and % specific lysis calculated.
Several advantages of CART cells, according to their features, include: (1) specific lysis; (2) duration in vivo; and (3) recognition of tumor cells in a MHC-independent manner.
The cell indexes of targets in the DVD-Ig protein treated samples were divided by the cell indexes of control targets (no treatment) to calculate percent specific lysis.
Oncolytic viruses (OVs) have the capacity to induce specific lysis of tumor cells and indirectly impact tumor growth via vascular shutdown.
Newcastle disease virus (NDV) has been previously shown to possess oncolytic activity, causing specific lysis of cancerous but not normal cells.
Specific lysis was determined after 48 hours.
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CEO of Professional Science Editing for Scientists @ prosciediting.com