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Significant differences in relative areas stained and mean specific intensity for the stains between control and treatment groups in mouse tissue were calculated.
Significant differences in relative areas stained and mean specific intensity for the stains between control and treatment groups in mouse tissue were tabulated.
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Specific mean fluorescence intensity (MFI) for NFκB1 (p50) was calculated by subtracting the non-specific MFI of staining with the isotype-matched control mouse IgG1.
Surface expression of E-CR1 and E-C4d on the gated erythrocytes was reported as specific mean fluorescence intensity (sMFI).
The specific mean fluorescence intensity for cells stained by each antibody is reported as the ratio of the CD18 to that of the negative control antibody.
The specific mean fluorescence intensity of the granulocyte and monocyte C5a receptor expression was calculated by subtracting the background mean fluorescence intensity obtained with the negative isotype control mAb from the value obtained with the anti-CD88 mAb.
The granulocyte and monocyte expression of the C5a receptor (CD88) was measured as specific mean fluorescence intensity of the whole population of granulocytes and monocytes, and as the relative amount of CD88-positive granulocytes and monocytes.
Luminescence was measured using a Luminex® instrument (Bio-Rad, Hercules, CA, USA), and the mean fluorescence intensity specific for each gene (proportional to the mRNA captured by the bead) was generated.
Specific mean fluorescence intensities (ΔMFI) were determined by subtracting the isotype-matched control antibody fluorescence.
b Mean signal intensity for specific brain regions normalized to muscle for WT and KO mice.
HSC70 and MHCII levels were expressed as the ratio of relative mean fluorescence intensity for specific antibody/mean fluorescence intensity for isotype-matched control antibody.
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