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(R -[C]PK11195 was synthesized R -[C]PK11195h a radiochemical purity >98% and mean specific activity of 95.7 ± 28.4 GBq/μmol, as described previously [ 28].
The determined mean specific activity of Cs for all the birds studied was very low in comparison with that for the bones of other Polish raptors studied up to the present [ 75].
One microgram of purified TrkA ECD proteins (Sino Biological Inc., Beijing, PRC) were incubated in 96-well plates with different concentrations of H-MT2 (0.12 μM–3 nM; mean specific activity 21.5 μCi/nmole) at 4° C for 10 min in 0.1 ml of binding buffer (50 mM Na-K phosphate), pH 7.1, 200 mM NaCl, in the presence or absence of 100-fold excess MT2.
The mean specific activity of 18 F-DPA-714 obtained was 113.9 ± 36.9 GBq/μmol.
The background specific activity was the mean specific activity in the phantom voxels outside these sphere VOIs.
The mean specific activity was 8.14 ± 3.8 MBq per microgram of protein, and the mean radiopurity was 98% ± 0.83% by instant thin-layer chromatography.
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The RCP and the decay corrected RCY for [68Ga]3 were measured to > 98% with mean specific activities of ~ 8 MBq nmol− 1.
Data shown are the mean specific activities from at least three independent BBMV batches from each strain measured in at least three independent experiments.
There was a difference in the mean specific activities of sTK1 from healthy dogs, dogs with CMT, and the dog with ALL, namely, 49 ± 21, 20 ± 10, and 760 ± 40 nmol/min/mg, respectively.
For no less than 90% specificity to recognize the positive candidate in this uricase pair, the mean of specific activity plus 1.4-fold of the SD of the starting material can be a practical threshold (Additional file 1: sheet 2: ROC analysis of activity).
All determinations were performed three times in triplicate and were presented as the mean ±1 specific activity of the SMase (SMase U/mg total protein/hr) was arbitrarily defined as 1 U = 1 DPM SMAase.
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