We briefly discuss the feasibility of using nematode skeletons obtained with MEME (Videos S2 to S7).
Pictures of different articulated Hypnomys cf. onicensis skeletons obtained at Cova des Pas de Vallgornera (Llucmajor, Mallorca) [28] were used to obtain direct body measurements (Figure 4).
After individual values of FA had been aligned, they were projected onto the mean skeleton.
Mean trabecular area, mean trabecular perimeter and mean skeleton length were calculated by total trabecular area, perimeter and skeleton length in ROI relative to trabecular number, respectively.
There was no significant difference between the two groups in apparent trabecular number, mean trabecular area, mean trabecular perimeter, mean skeleton length, total skeleton length, energy, absolute value, inverse difference, angular second moment and other parameters (p > 0.05).
In OA group, bivariate correlation analysis showed App BV/TV (r=−0.559), mean trabecular area (r=−0.940), mean trabecular perimeter (r=−0.574), mean skeleton length (r=−0.529) were associated with Euler number.
Apparent trabecular bone volume fraction (BV/TV), mean trabecular area, mean trabecular perimeter and mean skeleton length negatively correlated with Euler number in OA group.
Next, the thinned mean fractional anisotropy images were used to create a mean skeleton, representing the centres of all tracts common to the group.
Once the fibril skeleton was obtained, the endpoints of each skeleton were determined and used to calculate end to end distance.
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