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The vessel lumen area (mean size per square millimeter) and quantity of erythrocyte-containing vessels were determined using computerized image analysis software MicroImage analysis system (Casti Imaging).
Then, the size of each of the plaques was determined in pixels using Scion 1.63 (National Institutes of Health) and, finally, the mean size per sample was calculated.
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First, 452 Census Control Areas (CCA, mean size 1200 inhabitants per area) were randomly selected from complete list of all CCAs covering the entire country, proportionally to size of their respective Oblast (region).
For example, the average number of persons per household in the northern Manitoba region is 3.6, which is 44% greater than the mean size of 2.5 persons per household in Winnipeg as the largest urban centre in the same province.
The percentage of wild genome in the BC1F1 and BC2F1 generations and its relative diminution between the two generations, the mean size of wild introgression segments per LG and per generation, as well as the distribution of the wild segment lengths were estimated using the genotyping data available for each generation.
Data were collected on mean material usage per sequela occurrence until resolution (type of material = mean unit size per occurrence * mean frequency of use per unit).
The mean size of the litters of treated (12.3 offspring per litter) and control (10.6 offspring per litter) were similar.
The size analysis consisted of 30 measurements per sample, and the results are expressed as mean size ± SD.
However, macroscopic scoring of tumours at dissection showed significantly fewer (p = 0.025 Mann–Whitney), yet larger tumours in the Apc Min Hunk−/− cohort (mean number 21.4per animal +/− 2.7SEM, mean size 7.1 mm2 +/− 0.3SEM) compared to the Apc Min Hunk+/+ cohort (mean number 36.2 per animal +/− 4.8SEM, mean size 5.7 mm2 +/− 0.3SEM).
Direct standardization, based on the mean population size per 5-year age-group throughout the study period, was used to calculate the overall age-adjusted incidence in males and females.
Therefore, we reduced the mean sample size per cluster to 14.5 by random sampling within practice.
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