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Next, the mean shift between fluoroscopic and nuclear images was corrected for by incorporating the position of the x-ray detector in the x and y direction.
The position of the x-ray detector was estimated by calculating the mean shift between point sources on fluoroscopic images and nuclear images in the x and y direction.
As shown in Table 1, from the four correlated images (Fig. 4A D), the mean shift between these two centers was 488.5 ± 121.8 nm, which is close to the resolution (~460 nm) of the FM in the iCorr system, suggesting that the limitation factor of the correlation accuracy in the iCorr system is the resolution of its FM module.
Although HNF4A did not meet our strict threshold for significance in the primary analysis, it was ranked first in our secondary analysis using a test statistic that was more heavily weighted towards the mean shift between groups (see Methods).
A wholesale vertical shift away from zero indicates a global mean shift between replicates.
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Numbers represent the mean shifts between the original and normalized M values.
Mean shift tracking discriminates between a target model in frame and a candidate model in frame.
The time interval between mean shift and the latest sample point before mean shift.
No significant correlation was detected between mean shift and interspecies sequence conservation level or distance from stop codon or polyA signal for each microsatellite (|r|<0.1).
Finally, we tested the hypothesis that variation in egg size and year modifies the relationship between mean shift length and nest specific correlation coefficients, and reproductive success by including the corresponding interaction terms in our models.
The ICC is the preferred estimate of test-retest reliability over the Pearson's correlation as it adjusts for any systematic mean shift that may be present between visits.
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