Sentence examples for mean sequence quality from inspiring English sources

The Phred score was measured and the mean sequence quality >30 was estimated, exhibiting an accuracy of 99.9% (Additional file 1).

The percentage of reads with mean sequence quality > = Q30 was 94.32 ± 1.21, 96.76 ± 1.02, and 83.52 ± 1.58, respectively, indicating that the quality of the unmapped reads was lower than that of the uniquely mapped reads (Additional file 3: Table S2).

The algorithm considers the geometric mean of sequence qualities for every allele read to compute this probability [ 48].

For the raw FASTQ data collected from high-throughput sequencing, sequencing quality analysis was first performed using FASTQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) to ensure the mean values of sequence quality (Phred Score) for each base is greater than 32.

Reads were then filtered based on the sequence quality (mean read quality ≥ 30 and with < 10 Ns) and mapping quality (alignment score ≥ 98), and non-primary multiply mapped reads were removed.

The Q30 (means a sequencing quality score of 30, indicating a 0.1%% chance of an error, and thus 99.9 % confidence) percentage was 89.79 % and guanine-cytosine (GC) content was 51.45 % on average.

In brief, low-quality reads with a quality score < Q20 (means a sequencing quality score of 20, indicating a 1%% chance of an error, and thus 99%% confidence) were discarded and all SLAF pair-end reads with clear index information were clustered depending on sequence similarity by using BLAT (−tileSize = 10 -stepSize = 5).

We found that 3,977 KRM2 60-mer probes matched rhesus STS sequences with a mean similarity of 99.4%, demonstrating high sequence quality in 3' UTR probe design regions despite the overall low 3.5× genome coverage (R. A. Gibbs personal communications) in the January 2005 build of the rhesus genome.

Pyrosequencing of 16S rRNA amplicons from 161 fecal samples resulted in an average of 3,423 sequences per sample after quality control (551,183 sequences in total) with a mean sequence length of approximately 490 bp.

Mathematically, this can be understood by noting that the expected sequence quality

Sequence quality differed between the two sequencing lanes with a mean phred score at base 64 of 26.7 in library one and a mean phred score at base 64 of 33.6 in library two.