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The mean sequence coverage of the genomes were 23, 24 and 32 times respectively and the completeness of the genome sequences were estimated to be 98% for all isolates.
We achieved mean sequence coverage of 111× for this genome.
Supporting Information Table 1 lists the mean sequence coverage by protein.
For ~80% of the clones, the best assemblies consisted of less than 10 contigs at 24-fold mean sequence coverage.
For example, BTN1, DMB2 and TAPBP were highly expressed, with mean sequence coverage per nucleotide of 34.6, 23.0 and 21.5, respectively (Table 1).
The 6505 proteins that meet this requirement are supported on average by over 22 distinct peptides per protein, and have a mean sequence coverage of >45%.
Similar(41)
The mean sequencing coverage across targeted bases was 95×, with 82% of target bases above 30× coverage.
These requirements were defined after joint consideration of reviewed references [ 43– 47] and the features (e.g. mean sequencing coverage) of our data.
If we assume that the genome size of black grouse is similar to that of chicken (1.05 G), the estimated mean sequencing coverage of the black grouse genome was 127X.
The average sequence coverage was 38.7-fold.
Individual G and C nucleotides had lower average sequencing coverage (mean = 111,750) compared to A and T nucleotides (mean = 114,853; t = 10.51, df = 13,765, p < < 0.001, Additional file 1: Figure S4), also when controlling for GC content of the surrounding region (Additional file 1: Table S1).
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