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The mean seeding efficiency of all scaffolds was 64.8% (median 64.1%, range 41.7% – 83.3%).
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Here, we demonstrated that the cell seeding efficiency of BMSCs in the Cytomatrix® scaffold can be improved significantly (t-test, p<0.05) by means of macroencapsulating the scaffold via the complex coacervation of a methylated collagen and terpolymer.
Cell seeding efficiency was greater than 95%.
Centrifugal seeding increases seeding efficiency and cellular distribution of bone marrow stromal cells in porous biodegradable scaffolds.
Significantly higher cell seeding efficiency was found on composite scaffold.
Quantification of seeding efficiency confirmed increased adhesion of tumor cells into mineralized scaffolds (Fig. 2B).
Zonal seeding efficiency also demonstrated that chondrocytes were preferentially attached based on the seeding protocol with 9 16% of chondrocytes seeded on the fibre membrane, 66 68% seeded in the particulate-templated scaffold, yielding a total seeding efficiency range of 75 84%.
No significant difference in the seeding efficiency was observed among the scaffold groups indicating that microbead incorporation did not cause any difference in seeding efficiency.
According to the results of single substitutions described above, L138M substitution improves seeding efficiency and S135N, N143S, M129L, and I139M decrease seeding efficiency.
Seeded scaffolds were cultured for up to 7 days and assessed for seeding efficiency, proliferation and chondrocyte distribution.
Seeding efficiency was calculated as the percentage of cells in the scaffold from the total number of seeded cells.
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