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For all constructs, mean run lengths exhibited significant asymmetry, depending upon the direction of load.
Mean run lengths and run velocities for individual RNPs were also highly correlated.
The relatively short mean run lengths are consistent with the previously published mean run lengths for 1V:1VI competition experiments along 1D actin filaments, albeit with quantum dot scaffolds (Ali et al., PNAS, 2011).
Therefore, a direct comparison of mean run lengths in the 2 actin tracks is not very meaningful.
(A ) Mean run lengths per RNP for HLE and h SL1x3 RNPs with and without kin401T99N:mGFP.
There was, however, a subtle increase in both minus and plus end mean run lengths and velocities with increasing numbers of SL1 elements.
Similar(37)
Also, the other multi-site deficit run characteristics namely, the number of runs, the maximum run length, the mean run sum and the mean run length are well preserved by the AMHMABB model.
The increase of the step/turn ratio in starved cells strongly influences the length of the nearly straight runs; the mean run length increased from 2.1 pseudopodia in vegetative cells (1 turn and 1.1 steps) to 7.0 in starved cells (1 turn and 6.0 steps).
(D ) Mean run length per RNP for each RNA species.
(C ) Mean run length per RNP of motile h WT or HLE RNPs.
(B – E ) Correlation analysis of mean run length and run velocity for the bidirectional subset of h WT RNPs.
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