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Surprisingly, YanI in KF solution shows a lower mean reversal potentials (−19.2 ± 1.6 mV, n = 4 patches), while in KNO3 solution displays a much negative mean reversal potentials (−52.3 ± 0.4 mV, n = 4 patches) in comparison with that of in KCl solution.
YnaI in asymmetric KCl solutions (150 mmol/L/15 mmol/L) exhibited a mean reversal potential of −39.2 ± 0.5 mV (n = 5 patches), corresponding to a cation-to-anion (PK PCl) permeability ratio of about 9 1.
Indeed, YnaIM158A in the TM region alters the mean reversal potential from −39.2 ± 0.5 mV to −20 ± 0.5 mV, corresponding to decreased cation-to-anion (PK PCl) permeability ratio from 9 1 to 3 1 (Fig. 3A C).
The mean reversal potential was −43±11 mV (N = 8).
The mean reversal potential was also in the same range (mean Einv (FFA) ± SEM = −10.43±2.31 mV, n = 3).
Figure 5 demonstrates that this is indeed the case (group data is normalized to the overall mean reversal rate for an observer).
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