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Mean relative quantities were calculated for each paired tumour and normal tissue sample and a paired Student's t test was performed to compare groups.
It showed that the mean relative quantities of tau protein in the brains of 263K- or 139A-infected hamsters were significantly higher than those of the healthy ones.
The mean relative quantities of p-tau (Ser202/Thr205) in the infected hamster brains were significantly higher than that in the normal controls (P < 0.001, Figure 4B).
Scanning the image of each blots identified the mean relative quantities of p-tau (Ser396 and Ser404) in the infected hamsters were remarkably lower than that in the control group (P < 0.001, Figure 3).
Similar(56)
Data were normalized to 5s rRNA expression and are given as the mean relative quantity (compared with control), with error bars representing the standard error of the mean.
Data were normalized to Gapdh expression and are given as the mean relative quantity (compared with control), with error bars representing the standard error of the mean.
The fold changes were calculated by dividing the mean of the relative quantities (RQs) for the T17M RHO mice by the mean RQ of the wild-type mice at each time point.
Normalization of gene expression was conducted using the geometric mean of the relative quantities of actβ and GAPDH (δδCt method, appendix 1).
If multiple, similar terms were available, a representative term was selected for inclusion in this table eQPCR mean fold-change was calculated as mean RA-Zoo relative quantity (RQ) divided by mean RA or RA-PH RQ for genes up-regulated in RA-Zoo, and as mean RA or RA-PH RQ divided by mean RA-Zoo RQ for genes down-regulated in RA-Zoo (indicated by a negative sign).
This means that only the relative quantities |ω ij | are important, not their absolute values!
Statistical analyses were performed on the quantification cycle (Cq) data, using the Wilcoxon matched pairs test, and the results were expressed as the mean ± standard error of the mean (SEM) of the log normalised relative quantities (NRQ).
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