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Mean relative quantification and 95% confidence intervals are shown in all figures for easier interpretation of results.
The mean relative quantification (RQ) of MMP-28 gene expression was found to be highest in gingival tissues of subjects with healthy periodontium (RQ = 0.97 ± 2.08).
Pair wise comparison of the three groups using Tukey's multiple post hoc procedure (Table 4) showed the mean relative quantification (RQ) of MMP-28 gene expression to be highest in gingival tissues of subjects with healthy periodontium (RQ = 0.97 ± 2.08).
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Relative gene expression was calculated by mean of relative quantification (2-ΔΔCt) as previously described [ 60].
Analysis of SMARCB1/INI1 copy number by real-time quantitative PCR was performed by means of relative quantification using standard curve method [ 32], using TaqMan assays 4401631 (Applera) for RPPH1 endogenous control gene and Hs01497967_cn (Applera) for SMARCB1/INI1.
The presence of SMARCB1/INI1 hemyzigous deletion was confirmed also by Real-time quantitative PCR analysis of SMARCB1/INI1 copy number by means of relative quantification using standard curve method (not shown).
The arithmetic mean (with the standard deviation) and median relative quantification of THADA gene expression in thyroid normal tissue, hyper- and neoplasias are listed.
Correlations between different mean ΔCt values and relative quantification of miRNA expression were assessed using the Mann–Whitney test for two unpaired groups.
Vial activities >400 MBq had very low relative quantification errors (mean +0.29%, median +0.72%, 95%CI-3.30%to to +3.87%), whereas vial activities <400 MBq had larger errors (mean +4.35%, median +4.95%, 95%CI-8.43%3% to +17.12%; Additional file 1: Figure S2) - a finding attributable to background noise from 176Lu within the LYSO crystal [31, 32].
For relative quantification, the mean of duplicates was used.
Based on relative quantification method (mean fold change >2 or <2), bioinformatical analyses, we determined that miRNA374B (−3.38-fold), miRNA374A (−2.1-fold), and miRNA150 (−4.1-fold) were decreased in treated U87 cells versus control groups.
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