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Results of six replicates are expressed as the mean relative light units (R.L.U).
The mean optical density (490 nm) or the mean relative light units (RLU) obtained with the PPD+/PPD− healthy individuals + 3 standard deviations (SD) was used as cut-off to determine positive reactivity.
The activity is represented as mean relative light units per 5 × 104 epimastigotes.
Data were calculated as a ratio of mean relative light unit (RLU) for the sample to the mean RLU values for the assay of a positive calibrator (PC).
CellTiterGLO assay was performed on days 1, 4, and 7 to measure cell viability (mean relative light units [RLU] ± SD, n = 3).
In contrast to these fold inductions, which were derived from standardised mean relative light units (Firefly LUC values standardised by expression of Renilla LUC), there was no significant difference between the SARE-driven firefly LUC values in 0.5% and anoxia.
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Luminescence was measured by a microplate reader (Hidex, Mustionkatu, Turku, Finland), and the results are presented as a mean of relative light units (RLU) ± SD (n = 4).
ANOVA: analysis of variance; β-gal: β-galactosidase; CAG: chicken β-actin; Dox: doxycycline hydrochloride; E: embryonic day; EGFP: enhanced green fluorescent protein; GFP: green fluorescent protein; IHC: immunohistochemistry; P: postnatal day; PBS: phosphate-buffered saline; SEM: standard error of the mean; RLU: relative light units; tet: tetracycline; tTA: tetracycline-dependent transactivator.
Specific infectivity was calculated by subtracting the mean Env-pp relative light unit (RLU) signal from the HCVpp signal.
Average luminescence (in relative light units; RLU) was divided by the mean RFU obtained for the same well from using the CellTiter-Fluor kit (Promega).
Syk knockdown was confirmed by FACS analysis: Mean intensity of controls was 409 (relative light units), for Syk knockdown 262 indicating a 60% reduction of Syk surface expression after subtraction of auto fluorescence (177).
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mean relative amplitude
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mean daytime light
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mean maximum light
mean ambient light
mean relative humidity
mean relative branch
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