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The fold change in mean relative copy numbers was 73.2.
The fold change in the mean relative copy numbers between high USPC TF expressers vs NECs was 8.7 (P=0.01).
Values from this curve were used for normalization, so that each time point had a mean relative copy number equivalent to the proportion of the genome replicated at that time point.
The relative copy number of EPOR using each primer/probe set is the ratio of the copy number at the EPOR locus to the copy number at the control locus; the mean relative copy number is presented.
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(B ) Scatter plot of average relative copy numbers.
Group means with 95% confidence intervals (CIs) were calculated by computing them on ΔCTs and reverse transforming the results to obtain means (95% CIs) of relative copy numbers.
Group means with 95% confidence limits (CIs) were calculated by computing them on the Δ Cts and then reverse-transforming the results to obtain means (95% CIs) of relative copy numbers.
Group means with 95% confidence intervals (CIs) were calculated by computing them on the ΔCts and then reverse transforming the results to obtain means (95% CIs) of relative copy numbers.
Group means with 95% confidence limits (confidence intervals) were calculated by computing them on the ΔCTs and then reverse transforming the results to obtain means (95% confidence intervals) of relative copy numbers.
Final result is presented as relative expression of the gene relative copy number compared to the mean of the same gene in PBS control group.
For these populations, we also found a massive duplication of FFAR2 (fig. 1) with a copy number close-similar to that found in the European domestic line (relative copy number of 23.0 ± 1.5 (mean ± SEM; n = 4).
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