Pokkali and two RILs were declared as failed samples for having less than 10%% of the mean reads per sample.
Mean and median mRNA half-lives, mean reads per kilobase per million mapped reads (RPKM) values, and mean mRNA lengths were calculated for each chromosome individually and the whole set of autosomes.
Because of high sequence read coverages, 153, 379, and 273 mean reads per site for Klebsormidium, Mesotaenium, and Roya, respectively, no gaps or ambiguous regions were present (supplementary fig. S1, Supplementary Material online).
Mean reading time per case and its standard deviation was computed for every reader in both reading modes.
Subjects in the study of Goodrich et al.[ 16], were comparable to subjects in the present study and, to our knowledge, this was the only study that provided a mean reading speed in words per minute including standard deviations at the beginning and end of training in the use of CCTVs.
e Mean read coverage per genomic base.
Median contig length and the mean read number per contig were 226 bp and 25.5, respectively.
The mean read depth per base of each sample by Illumina short reads ranges from 14 and 79× (Additional file 1: Table S1).
The number of contigs varied from 110 to 578 across libraries and mean read depth per contig varied from 2.8 to 4.9 sequences (Table 2; Figure 2).
The results following alignment show that 86.9% of all the PKW gDNA reads successfully mapped to the A-genome, with a mean read coverage per reference A chromosome, of 41.4x (Table 1).
Genome sequencing of the isolates (except for Mu_DL045) was performed using an Illumina GAIIx DNA sequencer with 36, 76, or 100 cycle paired-end chemistry, generating read lengths of 35 101 nucleotides and 16-240x mean read depth per isolate.
